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        本公司正在利用高通量混库方法构建玉米CRISPR/Cas基因编辑突变体库,以QTL候选区间上千个基因为目标的第一阶段已经有所规模,共计获得了800余基因的数千转化事件,且数量依旧在不断增加。如此清单中含有您感兴趣基因,则可直接订购;如果不在当前清单中,我们也可提供任何数量的从头基因编辑服务。

        您可在此输入基因号(如Zm00001d012206或GRMZM2G095209)进行查询。若输入KN5585,将展示库里目前所有基因。如果需要获取种子,请发送基因号及订单号到sales@wimibio.com,我们将根据不断更新的种子情况与您进一步确认。每个突变体事件将提供10~30粒种子,如果库存少于50粒,我们将在繁种后送出。在最终清单得到确认后,请填写MTA表格(下载),提供pdf电子版到sales@wimibio.com. 一般情况下,每份突变体事件的种子将收取人民币6000元,如果一次性订购10份以上,则每份收费3000元费用;如数目较多或有其他合作意向,也可直接邮件联系。注意:我们并不能百分百确编辑的准确性。


The integration of high-throughput gene editing and genetic mapping has great potential in rapid functional gene cloning and validation. Here, we report the development of a CRISPR/Cas9-based editing platform adapted to high-throughput gene knockouts in maize.  As an initial stage, we focused on its application in functional gene identification by integrating over one thousand candidate genes corresponding to agronomic and nutritional traits, which are derived from genetic mapping and comparative genomic analysis. Current stage has covered over 800 genes with a total of thousands of events. With a long-term goal of editing the whole gene sets of maize, this list will be updated constantly.

    Please search the gene in which you’re interested (e.g. Zm00001d012206 or GRMZM2G095209), or you can obtain all the list by typing “KN5585” (the name of transforming receptor). You could order directly for those being exactly included in the current database; we also provide de novo gene editing services for any number of genes or elements.
To request the mutant seeds, please send the order number(s) or gene ID(s) to sales@wimibio.com. We will feed back the information that meet the distribution prerequisites (e.g. another crossing should be performed if the stock seed is less than 50), and please send the MTA form (download) in pdf format to sales@wimibio.com once the consulting list is confirmed. Each mutant event will only be provided with 10~30 seeds. A charge of US $1000 per event (many genes have more than one event) is required given the high investment in producing and maintaining the library; and the fee can be reduced to US $500 per event if more than 10 events are requested. Please feel free to contact us if you have a considerable amount of editing targets or other kinds of collaborative intentions.

Please NOTE:

      1.we are NOT responsible for ensuring 100% accuracy of (exclusively) targeting the given genes.
      2.It’s order’s responsibility to get an import permit from local governments if there’re any seed import restrictions.





 
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